1/ Purpose:
Learn why some substances passed across the membrane and other did not. Through that gain the knowledge to expain others real life experiences.
2/ Hypothesis:
- If the concentration inside the cell is higher than outside environment then some substances from the outside environment can pass across the cell membrane to get inside the cell.
- If the cell membrane doesn’t have the ability to avoid substances that maintain disease then through diffusion, the substances can get in the cell with the disease
- If lots of substances get into the cell can make the concentration inside the cell higher than the outside environment then some substances inside the cell might pass through the cell membrane to move out to the environment
3/ Materials:
- beaker
- onion skin
- dialysis tubing
- microscope
- string
- glass slide
- forceps
- starch solutions
- cover slip
- Iodine ( can stain your skin and is an eye irritant -> must wear safety goggles when using )
- bottle of salt water
- water dropper bottle
- teasing needle
4/ Procedure :
A – MAKE A “CELL”:
#1 Take a section of dialysis that has been soaking in water. Pull the ends apart, gently, forming a tube
#2 Seal one end of the tube by folding the end over and tying it closed with a piece of string or dental floss. The goal is to make that that end completely leak proof
#3 Pour the starch solution into the tube until it is about half-full
#4 Tide the top of the proof in the same way you tie off the bottom. The tube should not leak from either side. Check for leak by gently turning the tube to one end and back again, a few times.
#5 Rinse off the “cell” you have make my holding it under running water
#6 Place the “cell” in the beaker and add water until the “cell” is covered
#7 Put safety goggles over your eyes. Add iodine to the water in the beaker surrounding the “cell”. Add enough to make the water an amber color
#8 On your data sheet label the initial state diagram as directed
#9 Set the beaker aside and move to part B of this lab
B – OBSERVING AN ONION CELL
#1 After receiving the onion skin from the teacher position it in the center of a slide and place a drop of water on it. Be careful don’t let the onion skin fold over on itself. Use forceps and a teasing needle to get the onion skin flat.
#2 Add a cover slip and observe the cell using the low power of a microscope. Search for the cells with red coloration
#3 On your data sheet draw and color a typical onion cell mounted in water
#4 Remove the slide from the stage. Take the cover sheet off and bathe the onion cells in salt water. Take the cover slip back on the slide. Use the paper towel the plot the extra water.
#5 Place the slide back on the microscope and wait for a few minutes.
#6 While you are waiting, observe your “cell” sitting in the beaker. On your data sheet, label the final state diagram as directed.
#7 After completing the diagram, observe the onion cells in salt water.
#8 Draw and color an onion cell in salt water.
5/ QUESTIONS:
1/ Adding iodine into water can create a higher concentration environment out side the “cell” -> diffusion took place.
2 / The color inside the cell changed under the chemical reaction between iodine and starch solution.
3/ No starch diffused out of the “cell” because the water in the beaker still had the same color (while the water in the “cell” changed it color) -> the environment out side the “cell” was still the same (water and iodine)
4/ Iodine diffused through the membrane.
5/ The starch solution didn’t diffuse through the membrane.
6/ Some substances are able to pass through the membrane when others are not because dialysis tubing, like the cell membrane, is semi-permeable, which means it allows certain molecules or ions to pass through it by diffusion.
7/ The water inside the “cell” will not change its color (amber) while the water in the beaker changes its color into dark purple+blue.
8/ When the salt solution was added, an environment with higher concentration was created outside the onion cells -> osmosis took place -> the water inside the onion cells flow out.
9/ Organisms that contact directly with the salt can be badly damaged because the water inside cells of those will flow out the outside environment -> destroy the cell
10/
7/ OBSERVATIONS:
A – MAKE A “CELL”:
- The water in the beaker changed its color into amber after added iodine into.
- The cell changed its color into dark purple + blue after added iodine into the water.
B – ONION CELLS:
- Under the microscope the cell membranes and cell walls of the onion cells were seen.
- The onion cells increase sizes when added water and decrease sizes when added salt water.
8/ CONCLUSIONS:
A – MAKE A “CELL”:
The dialysis tubing played the role of the cell membrane in this experiment. Because it is semi-permeable, some certain substances can pass by it while others can not through diffusion. The iodine (mixed with water) in the beaker created a higher concentration outside the “cell” while the starch solution (also mixed with water) created a lower concentration inside the “cell” -> diffusion took place -> iodine diffused into the “cell”, have chemical reaction with the starch solution, this caused the water inside the “cell” changed its color into dark purple + blue. The starch solution couldn’t diffuse out of the cell so it didn’t make any chemical reaction with the iodine in the beaker -> the water there still kept its same color.
Diffusion also takes places in real life. It happens every seconds in our bodies and other organs through the cell membranes whenever a higher and lower concentration are created inside or outside the cell. This is the way for substances, both good and bad get into the cells and make changes.
B – ONION CELLS:
In this experiment, salt solution was added into the onion cells. This created a higher concentration in the environment outside the cells -> osmosis took place. Osmosis is a type of diffusion in which water diffuses through a cell wall or membrane or any semi-permeable barrier from a solution of low solute concentration to a solution with high solute concentration.
So in this case the water inside the onion cells diffused out into the outside environment (which has higher concentration) -> causes the cells decreased sized.
When added water the balanced between the two environment in and out side of the cells were recreated -> the water outside the cells diffused back in through the cell membranes -> the cells got back into original sizes.
Osmosis can easily being found in real life’s activities. Example when people tries to salted the food in order to keep it longer, it’s a kind of osmosis which the goal is taking all of the water out of the food to keep it dry and save from all kinds of fungi.
9/ POSSIBLE ERRORS:
The dialysis tubing was not tightly tied or had holes -> some starch solution got out and got mixed with the iodine in the beaker.
-> Redo the experiment, make sure the dialysis is tied well and had no holes on it so nothing can get out.
Couldn’t see the changes of the onion cells through the microscope
-> Make sure you are having the right objective lenses and look for an area with lots of triangular shapes with dark barrier surrounds it and a small spot inside.
10/ RESOURCES: Wikipedia, Bio text book.

